Journal
INTERNATIONAL IMMUNOLOGY
Volume 19, Issue 6, Pages 719-732Publisher
OXFORD UNIV PRESS
DOI: 10.1093/intimm/dxm034
Keywords
antigen presentation/processing; antigens/peptides/epitopes; dendritic cells; rodent
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Maturation of dendritic cells (DCs) regulates protein sorting in endosomal compartments to promote the surface expression of molecules involved in T cell activation. MHC Class II complexes are mobilized to the surface via intracellular effector molecules that remain largely unknown. We here show that protein kinase C (PKC) stimulates Class II antigen surface expression, using knock-in mice that express a Class II-green fluorescent protein fusion protein as a read out. Selective inhibition of PKC delta counteracts the ability of DCs to stimulate Class II MHC-restricted antigen-specific T cells. Activation of PKC does not affect antigen uptake, peptide loading and surface display of Class 1 MHC and transferrin receptor in DCs. We show that activation-induced Class 11 MHC surface expression is dependent on activation of PKC delta and conclude that this event is pivotal for optimal CD4 T cell activation.
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