Journal
ANALYTICAL METHODS
Volume 5, Issue 6, Pages 1542-1547Publisher
ROYAL SOC CHEMISTRY
DOI: 10.1039/c2ay26356d
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Funding
- Natural Science Foundation of Shandong Province [ZR2009BM029]
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This paper reports an approach for the direct determination of glutathione in individual Ramos cells based upon capillary electrophoresis coupled with electrogenerated chemiluminescence using a platinum electrode. The following conditions were suitable for the determination of glutathione: running buffer, 0.05 mol L-1 Na2HPO4-NaH2PO4 (pH 8.0); concentration of tris(2,2'-bipyridine) ruthenium(II) (Ru(bpy)(3)(2+)), 5.00 x 10(-3) mol L-1; injection voltage, 15 kV; injection time 13 s; separation voltage, 15 kV; detection potential, 1.2 V (vs. Ag/AgCl). Individual Ramos cells were injected into a fused-silica capillary by electromigration and lysed by 0.1% sodium dodecylsulfate. The concentration limit of detection of the method (S/N = 3) was 6.2 x 10(-7) mol L-1. The linear dynamic range was from 1.58 x 10(-6) to 2.00 x 10(-4) mol L-1 with correlation coefficient of 0.9967. The recovery was in the range of 97-103%, and the amount of glutathione in single Ramos cells ranged from 22.0-28.0 fmol. This method indicates a good reproducibility for single cell analysis to provide accurate analytical results.
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