Development of a simplified enhanced chemiluminescence enzyme linked immunosorbent assay (ECL-ELISA) for the detection of phosmet, azinphos-methyl and azinphos-ethyl residues in vegetable samples

A simple and sensitive enhanced chemiluminescence enzyme linked immunosorbent assay (ECL-ELISA) has been developed for the detection of phosmet (PMT), azinphos-methyl (APM) and azinphos-ethyl (APE) residues in vegetable samples. The concentration of the coating antibody and enzyme tracer as well as the effects of the pH value, ionic strength and organic solvent were studied. The measured sensitivities (IC50) of PMT, APM and APE were 8.56, 9.04 and 25.34 mu g L-1, respectively. High throughput screening for PMT, APM, and APE residues was achieved with good accuracy by means of the ECL-ELISA. The measurement time of the ECL-ELISA method was 5-10% shorter than that of the traditional ELISA method, and the sensitivity of detection was 2-3.5 times lower than that of the traditional method.