Biosensor for determination of hydrogen peroxide based on Yucca filamentosa membrane

A simple and low-cost hydrogen peroxide (H2O2) biosensor has been developed using a Yucca filamentosa plant leaf membrane in conjunction with an oxygen (O-2) sensor. The leaf membrane contains hydrogen peroxidase which can catalytically decompose H2O2 to produce O-2. The response rate was faster for a Yucca membrane (t(90) response time is approximate to 14 s) than a Yucca membrane with the O-2-permeable membrane (t(90) response time is approximate to 200 s) and the sensitivity was much improved. The biosensor exhibited an excellent linear calibration range from 85 mu M to 3.75 mM H2O2 (correlation coefficient r = 0.9999) with a detection limit of 15.1 mu M; repeatability (1.024%, n = 10). The effects of pH and temperature on the response of the H2O2 biosensor have been studied in detail. The working life-time of the biosensor was good as it retains 86.7% of its initial sensitivity at 25 degrees C even after 2350 determinations of H2O2 sample solutions. It has been successfully applied to the determination of H2O2 concentration in some commercial samples.