Journal
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
Volume 75, Issue 3, Pages 609-618Publisher
SPRINGER
DOI: 10.1007/s00253-007-0850-y
Keywords
basidiomycetes; ligninolytic; SAGE; transcriptome; biodegradation
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To analyze the transcriptome profile during the initiation of manganese peroxidase (MnP) and lignin peroxidase (Lil?) production in Phanerochaete chrysosporium, we constructed long serial analysis of gene expression (LongSAGE) libraries. A total of 13,666 tags (the number of cumulative counted tags) that included 6,945 unique tags (the number of distinct tags) were isolated from the day-3 culture, which just started the enzymes production and was 24 It after veratryl alcohol addition and oxygen-purge into the culture (day-2 culture). A total of 12,402 tags that included 6,396 unique tags were isolated from the day-2 culture, in which the activity of enzymes is not detected. The comparison of the two libraries suggested that 38 genes showed significant (p <= 50.01) fourfold or greater upregulation; this included the MnP gene (mnp2, mnp3) and UP H8 gene. On the other hand, 43 genes showed significant (p <= 0.01) fourfold or greater downregulation. This LongSAGE analysis found many new candidate genes regulating the enzymes production.
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