Journal
ANNALS OF PLASTIC SURGERY
Volume 58, Issue 6, Pages 652-655Publisher
LIPPINCOTT WILLIAMS & WILKINS
DOI: 10.1097/01.sap.0000250896.42800.a2
Keywords
lymphedema; indocyanine green; lymphography; near-infrared fluorescence; rats
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Lymphedema is known to be caused by many pathologic conditions; however, its diagnostic and therapeutic strategies remain to be unestablished. In this study, we investigated the usefulness of a novel lymphographic method based on fluorometric sensing using indocyanine green (ICG) dye for imaging lymphatic vessels using rat models. The real-time imaging system enabled visualization of superficial lymphatic vessels with a diameter of 0.1 mm in 33 frames/second. In addition, morphologic changes in lymphatic vessels in a radiation-induced lymphedema model were detected even at the latent stage. These results suggest that this imaging technique is acceptable as an evaluation method for the lymphatic system.
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