Journal
ANALYTICAL METHODS
Volume 4, Issue 6, Pages 1699-1703Publisher
ROYAL SOC CHEMISTRY
DOI: 10.1039/c2ay25106j
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Funding
- NSF China [21072162, 30830092, 30921005, 91029304, 81061160512]
- Fundamental Research Funds for the Central Universities [2011121020]
- 973 program [2009CB522200]
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Fluorescent imaging of mitochondria is an essential tool for studies of mitochondrial functions. The staining of mitochondria with potential-indicating dyes, e. g., rhodamine 123, readily vanishes upon loss of the transmembrane potential under certain conditions. 1-(Rhodamine B)-4-(2'-chloroacetyl)piperazine amide (RB-CAP) was shown to be electrophoretically accumulated into mitochondria, forming covalent bioconjugates with intramitochondrial protein sulfhydryls which enabled the mitochondrial staining to endure in subsequent collapse of the transmembrane potentials. RB-CAP is highly photostable and exhibits stringent selectivity in covalent labeling of mitochondria in living cells. Being much less expensive, RB-CAP is a superior substituent for MitoTracker probes in functional studies of mitochondria.
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