Journal
ANALYTICAL METHODS
Volume 4, Issue 5, Pages 1377-1382Publisher
ROYAL SOC CHEMISTRY
DOI: 10.1039/c2ay05828f
Keywords
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Funding
- Innovative Talent Training Project [CDJXS11220004]
- Chongqing University
- Natural Science Foundation of Chongqing [CSTC 2011BB5134]
- National Natural Science Foundation of China [NSFC81101417]
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This work takes advantage of electrochemically anodic pretreated carbon paste electrode modified with acetylene black nanocarbon particles (AB/CPE), a new and high-sensitive analytical method for insulin was put forward. Modern voltammetric testing techniques were used for primarily investigating the redox electrochemical characterization of Fe(CN)(6)(3-/4-) and electrochemical behavior of insulin on nanocarbon electrode surface. At the same time, a plausible mechanism was also proposed to provide insights into understanding how to facilitate the electron transfer between insulin biomolecule and electrode surface. The results have shown that the pretreated AB/CPE represented high accumulation efficiency to insulin and promoted its direct electron transfer rate owing to the presence of nanocarbon particles and anodic pretreatment. It is found that insulin exhibited a very sensitive anodic peak at 0.47 V on the pretreated AB/CPE, and its peak current was increased about six times more than that on the pretreated carbon paste electrode (CPE). A linear relationship between the anodic peak current and the concentration of insulin from 20 to 1000 nM and a limit of detection as low as 5 nM were obtained using the pretreated AB/CPE. Attracting attention, the proposed method was applied to the realistic samples successfully and showed good recovery and reproducibility.
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