Journal
COLLOID AND POLYMER SCIENCE
Volume 285, Issue 9, Pages 1055-1060Publisher
SPRINGER
DOI: 10.1007/s00396-007-1660-6
Keywords
core-shell type nanogel; pH-sensitive volume phase transition; endosomolytic agent; gene vector; PEG-tethering surface
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A pH-responsive PEGylated nanogel was successfully prepared by means of emulsion copolymerization of 2-(N,N-diethylamino)ethyl methacrylate (AMA) with heterobifunctional poly(ethylene glycol) (PEG) bearing a 4-vinylbenzyl group at the alpha-end and a lactose moiety at the omega-end in the presence of potassium persulfate and ethyleneglycol dimethacrylate as a cross-linker. Polyplex micelle composed of PEG-block-poly(L-lysine) copolymer and plasmid DNA (PEG-b-PLL/pDNA) exhibited a far more efficient transfection ability in the presence of lac-nanogel-8k-1.0% (PEG, M-n = 8000; cross-linking density, 1.0%) than the PEG-b-PLL/pDNA polyplex micelle alone (in the absence of lac-nanogel-8k-1.0%), suggesting that an appreciable fraction of lac-nanogel-8k-1.0% along with the PEG-b-PLL/pDNA polyplex micelle is taken up into the HuH-7 cells through the asialoglycoprotein receptor-mediated endocytosis process mediated by the cluster of a large number of lactose moieties on the surface of lac-nanogel-8k-1.0%, followed by the effective disruption of the endosome by the buffer effect of the unprotonated PAMA core in lac-nanogel-8k-1.0%.
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