Journal
ARCHIVES OF ORAL BIOLOGY
Volume 52, Issue 6, Pages 544-551Publisher
PERGAMON-ELSEVIER SCIENCE LTD
DOI: 10.1016/j.archoralbio.2006.12.002
Keywords
genioglossus muscle; obstructive sleep apnoea; myosin heavy chain; citrate synthase
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Objective: To examine whether short-term exogenous activation of a tongue muscle induced a phenotypic shift from a fast to a slow fibre-type, and thus assess a potential therapeutic avenue to protect against obstructive sleep apnoea (OSA). Methods: New Zealand White rabbit genioglossus (GG) muscle, characteristically a fast muscle, was continuously stimulated at a frequency attributed to slow muscle (10 Hz, 3 V DC pulses) using an implanted micro-circuit for 7 days. Changes in muscle fibre types and aerobic capacity were assessed between stimulated and un-stimulated (control) groups using immunohistochemistry and electrophoresis for myosin heavy chain (MHC) and assayed for citrate synthase. Results: Compared to the un-stimulated control group, stimulated GG muscles had more (similar to 13%) type I MHC (slow-twitch) content; a proportional decrease in type 11 MHC (fast-twitch) isoform also occurred in the stimulated GG muscle (P < 0.05). Electrophoresis analysis on whole muscle and single fibre MHC showed an increased type I expression in the stimulated GG muscle (P < 0.01). A commensurate rise in citrate synthase activity, indicating a change in aerobic capacity, was also observed in the stimulated GG muscles. Conclusion: Together, these results demonstrate a successful alteration in tongue muscle characteristics using exogenous electrical stimulation and perhaps a potential therapeutic application for OSA. (C) 2007 Elsevier Ltd. All rights reserved.
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