4.6 Article

Matrix-localization for fast analysis of arrayed microfluidic immunoassays

Journal

ANALYTICAL METHODS
Volume 4, Issue 10, Pages 3466-3470

Publisher

ROYAL SOC CHEMISTRY
DOI: 10.1039/c2ay25485a

Keywords

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Funding

  1. Ministry of Science and Technology [2009CB930001, 2011CB933201]
  2. National Science Foundation of China [51073045, 21025520, 20890020, 90813032, 21105018]
  3. Chinese Academy of Sciences [KJCX2-YW-M15]
  4. Novo Nordisk-CAS Research Foundation [NNCAS-2010-5]

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High-throughput assays necessitate high-throughput data analysis. Arrayed microfluidic immunoassay shows the capability of high-throughput protein detection. However, its development was restricted by the low efficiency of downstream data analysis. We present herein programming-based image processing through the local recognition of a sub-array followed by the region-growing algorithm to achieve fast, convenient and precise extraction of information with reduced personal bias.

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