Journal
ANALYTICAL METHODS
Volume 4, Issue 2, Pages 460-466Publisher
ROYAL SOC CHEMISTRY
DOI: 10.1039/c1ay05481c
Keywords
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Funding
- Natural Science Research Foundation of China [21175058, 51003039]
- Natural Science Foundation of Shandong Province, China [ZR2011BQ019]
- Excellent Young and Middle-Aged Scientist Reward Foundation of Shandong Province [2008BS09006]
- Technology Development Plan of Shandong Province, China [2011GGB01153, 2009GG20003022]
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A novel method for the determination of bovine serum albumin (BSA) is proposed based on the electrochemiluminescence (ECL) of BSA with CdTe quantum dots (QDs) films. Thioglycolic acid (TGA) is used to cap CdTe colloidal solutions to obtain stable water-soluble QDs and intensive anodic ECL emission with a peak value starting from +1.2 V (vs. Ag/AgCl) in a carbonate bicarbonate buffer solution (pH 9.32) at an indium tin oxide (ITO) electrode. The ITO glass is modified with CdTe QDs by the layer-by-layer (LbL) self-assembly technique, which is immobilized into a homemade ECL flow chip and used as a receptor of the ECL sensor. The ECL intensity is correlated linearly with the concentration of BSA over the range of 1.0 x 10(-9)-1.0 x 10(-6) g mL(-1) and 1.0 x 10(-6)-1.0 x 10(-5) g mL(-1), and the detection limit is 5.48 x 10(-10) g mL(-1). The relative standard deviation is 1.62% for 4.0 x 10(-7) g mL(-1) BSA (n = 11). This simple and sensitive method reveals good reproducibility for ECL analysis. It puts forward a new efficient ECL method for the determination of BSA and opens new avenues for the application of QDs in ECL biosensors.
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