Journal
ANALYTICAL METHODS
Volume 3, Issue 9, Pages 2127-2133Publisher
ROYAL SOC CHEMISTRY
DOI: 10.1039/c1ay05164d
Keywords
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Funding
- EU [224306]
- NERC
- EPSRC/NERC [EP/E016774/1]
- EPSRC [EP/E016774/1] Funding Source: UKRI
- NERC [noc010003, noc010013] Funding Source: UKRI
- Engineering and Physical Sciences Research Council [EP/E016774/1] Funding Source: researchfish
- Natural Environment Research Council [noc010003, noc010013] Funding Source: researchfish
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Development of integrated systems for nucleic acid analyses is mainly driven by the requirement for fast and simple clinical and environmental diagnostics. The need for affordable and effective point-of-care diagnosis has inspired an entire field of biotechnology in micro and nano-fluidics. We are developing a microfluidic system that has individual sub-systems for performing cell concentration and lysis, RNA extraction/purification and real-time RNA detection. The system is being developed to analyse the rbcL gene of phytoplankton Karenia brevis, a species responsible for harmful algal blooms. This integrated system will perform sub-cellular analysis of RNA using nucleic acid sequence-based amplification and would be used in large scale biochemical analysis and experimentation. The device could potentially be used for the detection of any species with a known target nucleic acid sequence for in situ environmental monitoring, forensics or clinical diagnostics.
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