Journal
ANALYTICAL METHODS
Volume 2, Issue 9, Pages 1236-1242Publisher
ROYAL SOC CHEMISTRY
DOI: 10.1039/c0ay00284d
Keywords
-
Funding
- National 863 Program [2007AA03Z357]
- National Natural Science Foundation of China [20975026]
- Shanghai Key Basic Research Program [08JC1402600]
- Research Fund for the Doctoral Program of Higher Education [200802461096, 20090071110056]
Ask authors/readers for more resources
A new concept is proposed in this article to detect multiple cancer markers in a single sample. Quantum dot (QD) fluorescence (FL) labels were successfully combined with enzyme chemiluminescence (CL) labels for simultaneous detection of three cancer markers in human serum using just a common 96-well plate reader and with equal detection limits for the three markers. As a proof-of-concept, herein we coupled one QD FL label with two enzyme CL labels for hybrid multiplexed detection of lung cancer markers as exemplified by neuron-specific enolase (NSE), carcinoembryonic antigen (CEA) and cytokeratin fragment (Cyfra21-1). A homogeneous sandwich-type detection strategy was employed herein, where the bead-antibody mixture first reacts with NSE, CEA and Cyfra21-1 to initiate three immunoreactions in a single tube; and then the formed conjugates sandwiches with biotin, digoxin and fluoresceinisothiocyanate (FITC)-modified detection antibodies, and further reacts with a mixture of streptavidin QD, anti-FITC horseradish peroxidase (HRP) and anti-digoxin alkaline phosphatase (ALP) for subsequent CL and FL detection. The results show that NSE, CEA and Cyfra21-1 could be sensitively determined with a common 96-well plate reader and with equal detection limits down to the ng mL(-1) level. Furthermore, the proposed method has been successfully applied to the determination of three cancer markers in human samples without cross-reaction. Because it is straightforward to adapt this strategy to detect a spectrum of other proteins by using different antibodies or aptamers, this new CL strategy might create a universal technology for developing simple biosensors in the sensitive and selective detection of multiple targets in a variety of clinical, environmental, and biodefense applications.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available