Journal
AMERICAN JOURNAL OF HEMATOLOGY
Volume 82, Issue 6, Pages 463-472Publisher
WILEY
DOI: 10.1002/ajh.20707
Keywords
stem cell; marrow; cryopreservative; freezing methods
Categories
Funding
- NCI NIH HHS [L30 CA104092-01, L30 CA104092-02] Funding Source: Medline
- NCRR NIH HHS [P20RR018757, P20 RR018757, P20 RR018757-035855] Funding Source: Medline
- NIDDK NIH HHS [K0 DK064980, R01 DK61858, K08 DK064980, R01 DK061858] Funding Source: Medline
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Stem cell transplantation represents a critical approach for the treatment of many malignant and non-malignant diseases. The foundation for these approaches is the ability to cryopreserve marrow cells for future use. This technique is routinely employed in all autologous settings and is critical for cord blood transplantation. A variety of cryopreservatives have been used with multiple freezing and thawing techniques as outlined in the later chapters. Freezing efficiency has been proven repeatedly and the ability of long-term stored marrow to repopulate has been established. Standard approaches outlined here are used in many labs as the field continues to evolve.
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