DEVELOPMENT OF NEAR-INFRARED REFLECTANCE SPECTROSCOPY MODELS FOR QUANTITATIVE DETERMINATION OF WATER-SOLUBLE CARBOHYDRATE CONTENT IN WHEAT STEM AND GLUME

Water-soluble carbohydrate (WSC) reserved in stem is an important agronomic trait for crop improvement. The intact samples and pieces of chipped samples were employed to determine WSC content by near-infrared reflectance spectroscopy (NIRS). Three NIRS models were developed to predict WSC content in wheat stem lower internode, upmost internode, and wheat glume, respectively. Moreover, a mixed model was developed for WSC quantitative analysis in the mixed sample of the three wheat organs. Statistics analysis indicated that the four models showed a high determination coefficient (R-2 >= 0.97) and ratio of standard deviation to RMSECV (RPD >= 5.99). The NIRS models would allow rapid and high throughout assessments and selections of WSC contents in wheat genetics and breeding programs.