Journal
ANALYTICAL LETTERS
Volume 44, Issue 9, Pages 1591-1601Publisher
TAYLOR & FRANCIS INC
DOI: 10.1080/00032719.2010.520391
Keywords
Antibody; coating hapten combination; Cross reactivity; ELISA; Monoclonal antibody
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Funding
- Key Projects in the National Science & Technology Pillar Program [2009BADB9B03]
- National High Technology Research and Development Program of China [2006AA10Z447]
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A new specific monoclonal antibody against the organophosphorous pesticide fenthion was produced based on our previous study. A sensitive and specific Enzyme-Linked Immunosorbent Assay (ELISA) for fenthion based on the new immunizing/coating hapten combination was developed. In this study, the H1 which attempts to expose the aromatic ring group was conjugated with bovine serum albumin (BSA) for the immunogen. All of the haptens that have been synthesized were conjugated with ovalbumin (OVA) for the coating antigen. The efficient antibody/coating conjugated combinations were selected, and the optimized ELISA was developed. In the optimized system, the IC50 value was 5.8ng center dot mL-1 with the detection limit (IC20) of 0.028ng center dot mL-1. The cross reactivity with all of the tested pesticides was lower than the 0.5%. Also, the system can detect the fenthion addition to the real samples such as soil, water, rice, and Chinese cabbages.
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