Journal
CHROMATOGRAPHIA
Volume 65, Issue 11-12, Pages 771-774Publisher
SPRINGER HEIDELBERG
DOI: 10.1365/s10337-007-0230-x
Keywords
column liquid chromatography; RP-HPLC-PDA; fingerprinting; glabridin in Yahsti-madhu; Glycyrrhizo glabra
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A reverse phase high performance liquid chromatographic method is developed for the quantitation of glabridin in Glycyrrhiza giabra, using C-18 column with acetonitrile-water containing 2% AcOH (70:30) as an eluent. Glabridin is detected by UV absorption at 280 nm after separation by the chromatographic system. Good linearity was obtained in the working range of the concentration (0.01-0.1 mg mL(-1)), with correlation coefficients 0.999. Limit of detection and limit of quantitation were 0.0195 and 0.065 mg mL(-1). The method was validated under ICH guidelines. The described method can be utilized for routine analysis (assays and stability tests) of G. glabra extracts and Ayurvedic medicine based on Yashti-madhu.
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