Journal
ANALYTICAL LETTERS
Volume 43, Issue 17, Pages 2773-2789Publisher
TAYLOR & FRANCIS INC
DOI: 10.1080/00032711003731464
Keywords
Enzyme-linked immunosorbent assay (ELISA); Fenvalerate; Hapten; Monoclonal antibody; Pyrethroid insecticide
Categories
Funding
- Program of National Science Foundation of China [30800771]
- Project in the National Science & Technology Pillar Plan [2009-BADB9B03-Z09]
- Chinese National '863' High-Tech. Research Program [2006AA10Z448]
- Science and Technology of Wuhan [200820337086, 200950431224]
- Chinese Agricultural Academy of Sciences
Ask authors/readers for more resources
Monoclonal antibodies (MAbs) against pyrethroid insecticide fenvalerate were achieved, identified, and applied in environmental water. Mice were immunized with a novel synthesized hapten conjugated with bovine serum albumin (BSA). Three positive clones of MAbs were obtained after cell fusion and hybridoma selection, among them MAb-2 (5B10) showed the highest reactivity toward fenvalerate. The IC50 of MAb-2 was 94.5ngmL-1; moreover, it showed lower cross-reactivity with other pyrethroids such as bifenthrin, tetramethrin, deltamethrin and beta-cypermethrin. Optimization of enzyme-linked immunosorbent assay (ELISA) was studied. The limit of detection (LOD) of the assay was 8.8ngmL-1 and the detection range was 0.017-27.33gmL-1. For preliminary application, addition recovery experiments in water samples were performed. The mean recoveries of three kinds of samples varied from 90.6% to 108.7% and the coefficients of variation ranged from 0.5% to 5.3%. The results showed that MAb-2 could be used for the detection of fenvalerate contamination in environmental water.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available