Microwave-Assisted Extraction and High-Performance Liquid Chromatography for Simultaneous Determination of Osthole and Isoimperatorin in Libanotis buethorimensis

Osthole and isoimperatorin are two of the major bioactive components in Libanotis buethorimensis, an effective traditional Chinese medicine. A method of microwave-assisted extraction followed by high performance liquid chromatography was developed for simultaneous determination of osthole and isoimperatorin in Libanotis buethorimensis. The conditions for extraction were formulated by using the response surface method, and the chromatographic condition was optimized. The calibration curves were linear from 10.0 to 800.0gmL-1 for osthole and from 1.0 to 200.0gmL-1 for isoimperatorin. Results showed that the proposed method is reliable and a potential tool for the quality assessment of Libanotis buethorimensis materials.