Journal
ANALYTICAL LETTERS
Volume 42, Issue 15, Pages 2389-2402Publisher
TAYLOR & FRANCIS INC
DOI: 10.1080/00032710903137376
Keywords
Affinity probes; aptamer; bioassay; Campylobacter jejuni; capillary electrophoresis; food pathogen
Categories
Funding
- Natick Soldier Center [DAAD16-00-C-09221]
Ask authors/readers for more resources
A qualitative capillary electrophoresis immunoassay was developed for the first-time to evaluate aptamer binding to bacterial cells. Binding affinity of aptamers developed against a Campylobacter jejuni bacterial cell target, relative to other common food-borne pathogens was investigated and specific binding affinity was evidenced by pronounced mobility shift and peak broadening with increasing bacteria concentration for both aptamers. Little to no mobility shift was observed for food-borne pathogens, Salmonella typhirium and Escherichia coli, even when increasing concentrations 10-fold over target. These results suggest that affinity probe capillary electrophoresis could be useful for qualitative screening of aptamer candidates for bacterial cell targets.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available