Journal
ANALYTICAL LETTERS
Volume 42, Issue 2, Pages 366-380Publisher
TAYLOR & FRANCIS INC
DOI: 10.1080/00032710802508149
Keywords
Amplification; biosensor; DNA; hybridization; magnetic composite; methylene blue; single-walled carbon nanotubes
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Funding
- National Science Foundation of China [20665003]
- Science and Technology Key Project of Ministry of Education of China [207087]
- Nature Science Foundation of Guangxi Province [0728214]
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A single-walled carbon nanotube (SWNT)/nano-Fe3O4/methylene blue (MB) magnetic composite was developed to fabricate the DNA biosensor. The magnetic SWNTs/nano-Fe3O4 and SWNTs/nano-Fe3O4/MB composites were prepared by chemical coprecipitation and adsorption, respectively. The morphology, infrared, and magnetic properties of different composites were characterized. The behavior of MB adsorbed in the composite matrix as indicator for the detection of DNA was studied via MB reductive current changes between after and before combination with DNA. Owing to the high electrical conduction of SWNTs and superparamagnetism of Fe3O4 nanoparticles, the biosensor exhibited simple operation, high sensitivity, and easy renewal. The biosensor was successfully applied to detect the hybridization of DNA.
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