Journal
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
Volume 16, Issue 4, Pages 7738-7747Publisher
MDPI
DOI: 10.3390/ijms16047738
Keywords
DNA technology; DNA microparticles; bioimaging; Forster resonance energy transfer (FRET)
Funding
- Global Innovative Research Center (GiRC) Program through the National Research Foundation of Korea (NRF) - Ministry of Science, ICT & Future Planning [NRF-2012K1A1A2A01056093]
- National Research Foundation of Korea [2012K1A1A2A01056093] Funding Source: Korea Institute of Science & Technology Information (KISTI), National Science & Technology Information Service (NTIS)
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Fluorescent labeling is widely used to investigate the structural stability and changes to DNA nano- and microstructures. Despite this, the conventional method for observing DNA structures has several limitations in terms of cost-efficiency. This paper introduces a DNA spherical particle stained with DNA intercalating dyes (SYBR Green and SYTOX Orange) as tracers and reports the interaction between multiple dyes. The interference between the dyes was analyzed in terms of Forster resonance energy transfer (FRET) and competition. The changes in the fluorescence intensity by FRET were uniform, regardless of the sequence. The competition effect could occur when several dyes were added simultaneously. These properties are expected to help in the design of multicolor tracers in bioimaging and environmental applications.
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