Journal
VIROLOGY
Volume 362, Issue 2, Pages 428-440Publisher
ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1016/j.virol.2006.12.034
Keywords
geminivirus; Beet curly top virus; Tomato golden mosaic virus; C4 protein; intracellular localisation; shaggy-related protein kinase; LRR-RLK; brassinosteroid signalling
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Beet curly top virus (BCTV) C4 interacted with two members of the shaggy-related protein kinase family (AtSK eta and AtSK zeta) and a putative leucine-rich repeat receptor-like kinase (LRR-RLK) in a yeast two-hybrid assay. Tomato golden mosaic virus (TGMV) AC4 also bound with similar efficiency to AtSK eta and AtSK zeta but was unable to interact with the LRR-RLK. BCTV C4 interaction with AtSK eta was confirmed using an in vitro binding assay. The protein kinases were capable of autophosphorylation in vitro and AtSK eta phosphorylated BCTV C4 at threonine and serine residues. AtSK eta phosphorylation of TGMV AC4 was significantly less efficient. The LRR-RLK did not efficiently phosphorylate BCTV C4. BCTV C4 localisation to the cell periphery in Nicotiana benthamiana was dependent on an intact N-terminal myristoylation motif, consistent with plasma membrane targeting. The intact motif was also required to produce the wild-type disease phenotype. Transient expression of BCTV C4 and TGMV AC4 derivatives in N. benthamiana identified additional amino acids within a central domain that contribute to the phenotype. The interaction with AtSK eta indicates that BCTV C4 interacts with the brassinosteroid signalling pathway. (C) 2006 Elsevier Inc. All rights reserved.
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