Journal
ANALYTICAL CHEMISTRY
Volume 90, Issue 18, Pages 10650-10653Publisher
AMER CHEMICAL SOC
DOI: 10.1021/acs.analchem.8b02699
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Funding
- National Institutes of Health [GM109682]
- NIH [AG046025, AI106100, AT008297, ES022360, ES023529, HHSN261201300033C]
- NATIONAL CENTER FOR COMPLEMENTARY & ALTERNATIVE MEDICINE [R43AT008297] Funding Source: NIH RePORTER
- National Center for Complementary & Integrative Health [R44AT008297] Funding Source: NIH RePORTER
- NATIONAL INSTITUTE OF ALLERGY AND INFECTIOUS DISEASES [R44AI106100, R43AI106100] Funding Source: NIH RePORTER
- NATIONAL INSTITUTE OF ENVIRONMENTAL HEALTH SCIENCES [R44ES023529, R43ES023529, R44ES022360, R43ES022360] Funding Source: NIH RePORTER
- NATIONAL INSTITUTE OF GENERAL MEDICAL SCIENCES [R43GM109682] Funding Source: NIH RePORTER
- NATIONAL INSTITUTE ON AGING [R44AG046025, R43AG046025] Funding Source: NIH RePORTER
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Direct quantitation of proteins in complex biological matrices by mass spectrometry remains a challenge. Here, we describe a novel top-down parallel reaction monitoring-mass spectrometry (PRM-MS) assay, enabled by microflow LC-nanospray MS using a silicon microfluidic LC-MS chip. We demonstrated direct analysis of intact proteins such as somatropin in human plasma, achieving sensitivity (0.1-1.0 fmole) and speed (1-5 min) on par with enzyme-linked immunosorbent assay (ELISA).
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