Journal
ANALYTICAL CHEMISTRY
Volume 90, Issue 17, Pages 10231-10238Publisher
AMER CHEMICAL SOC
DOI: 10.1021/acs.analchem.8b01644
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Funding
- Tom and Sondra Rykoff Family Foundation Research
- Israeli Centers of Research Excellence (i-CORE) Program on Plant Adaptation to Changing Environment
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Regardless of major advances in mass spectrometry imaging (MSI), there are three intrinsic limitations associated with MSI, including intricate molecular identification, low molecular coverage, and incapability to obtain true spatial distribution due to isobaric and particularly isomeric ions interference. We developed a novel approach that integrates in vivo dual isotope labeling of precursor metabolites with MSI (DLEMMA-MS-Imaging) for identification of spatially localized metabolite and metabolic network map reconstruction. In a proof-of-concept study, we identified 59 and 6 novel metabolites in lemna and tomato fruit, respectively. Significantly, 20-30% of the identified metabolites were found to contain at least one structural isomer that displays a different distribution pattern. The notable feature of this approach is the ability to differentiate localization of structural isomers, hence, providing the true distribution of molecules of interest.
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