Journal
ANALYTICAL CHEMISTRY
Volume 90, Issue 17, Pages 10286-10292Publisher
AMER CHEMICAL SOC
DOI: 10.1021/acs.analchem.8b02021
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Funding
- National Natural Science Foundation of China [21627807, 21705091]
- National Key Research and Development Program of China [2016YFF0100301]
- U.S. National Institutes of Health [R44GM119584]
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The presence of carbon carbon double bonds (C=Cs) in unsaturated phospholipids is closely related to lipid conformations and physiochemical activities. Previously, we have demonstrated that epoxidation reaction facilitated by low-temperature plasma (LTP) enabled the structural analysis of unsaturated fatty acids (FAs). Epoxidation of the C=C leads to the production of an epoxide, which can be easily cleaved via collision-induced dissociation (CID) to produce diagnostic ions indicative of the C=C bond locations in FAs. In this work, we further developed this method for analysis of phospholipids. Tandem mass spectrometry analysis with epoxidation reaction was performed in both positive and negative ion mode to analyze phosphatidylcholines (PCs), phosphatidic acids (PAs), phosphatidylethanolamines (PEs), phosphatidylglycerols (PGs), and phosphatidylinositols (PIs). The developed method was applied in a shotgun lipidomics approach to characterize phospholipids in a bovine liver extract.
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