Journal
ANALYTICAL CHEMISTRY
Volume 90, Issue 16, Pages 9983-9989Publisher
AMER CHEMICAL SOC
DOI: 10.1021/acs.analchem.8b02428
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Funding
- National Natural Science Foundation of China [21722503]
- 973 Program [2015CB659400]
- PAPD Program
- Shuangchuang Program of Jiangsu Province
- Open Funds of the State Key Laboratory of Analytical Chemistry for Life Science [SKLACLS1704]
- Open Funds of the State Key Laboratory of Coordination Chemistry [SKLCC1819]
- Fundamental Research Funds for the Central Universities [021314380103]
- Thousand Talents Program for Young Researchers
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The detection of phosphates and their enzymatic hydrolysis is of great importance because of their essential roles in various biological processes and numerous diseases. Compared with individual sensors for detecting one given phosphate at a time, sensor arrays are able to discriminate multiple phosphates simultaneously. Although nanomaterial-based sensor arrays have shown great promise for the discrimination of phosphates, very few of them have been explored for probing phosphates involved enzymatic hydrolysis. To fill this gap, herein we fabricated two-dimensional-metal-organic-framework (2D-MOF)-nanozyme-based sensor arrays by modulating their peroxidase-mimicking activity with various phosphates, including AMP, ADP, ATP, pyrophosphate (PPi), and phosphate (Pi). The sensor arrays were used to successfully discriminate the five phosphates not only in aqueous solutions but also in biological samples. The practical application of the sensor arrays was then validated with blind samples, where 30 unknown samples containing phosphates were accurately identified. Moreover, the sensor arrays were successfully applied to probing hydrolytic processes involving ATP and PPi that are catalyzed by apyrase and PPase, respectively. This work demonstrates a nanozyme-based sensor array as a convenient and reliable analytical platform for probing phosphates and their related enzymatic processes, which could be applied to other analytes and enzymatic reactions.
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