Journal
BIOCHEMISTRY
Volume 46, Issue 23, Pages 7054-7061Publisher
AMER CHEMICAL SOC
DOI: 10.1021/bi700467k
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Funding
- NHLBI NIH HHS [P01 HL62426, T32 HL07692, R01 HL077195, P01 HL062426, T32 HL007692, R01 HL083218] Funding Source: Medline
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Cardiac myosin binding protein C (cMyBPC) phosphorylation is essential for normal cardiac function. Although PKC was reported to phosphorylate cMyBPC in vitro, the relevant PKC isoforms and functions of PKC-mediated cMyBPC phosphorylation are unknown. We recently reported that a transgenic mouse model with cardiac-specific overexpression of PKC epsilon (PKC epsilon TG) displayed enhanced sarcomeric protein phosphorylation and dilated cardiomyopathy. In the present study, we have investigated cMyBPC phosphorylation in PKC epsilon TG mice. Western blotting and two-dimensional gel electrophoresis demonstrated a significant increase in cMyBPC serine (Ser) phosphorylation in 12-month-old TG mice compared to wild type (WT). In vitro PKC epsilon treatment of myofibrils increased the level of cMyBPC Ser phosphorylation in WT mice to that in TG mice, whereas treatment of TG myofibrils with PKC epsilon showed only a minimal increase in cMyBPC Ser phosphorylation. Three peptide motifs of cMyBPC were identified as the potential PKC epsilon consensus sites including a 100% matched motif at Ser302 and two nearly matched motifs at Ser811 and Ser1203. We treated synthetic peptides corresponding to the sequences of these three motifs with PKC epsilon and determined phosphorylation by mass spectrometry and ELISA assay. PKC epsilon induced phosphorylation at the Ser302 site but not at the Ser811 or Ser1203 sites. A S302A point mutation in the Ser302 peptide abolished the PKC epsilon-dependent phosphorylation. Taken together, our data show that the Ser302 on mouse cMyBPC is a likely PKCe phosphorylation site both in vivo and in vitro and may contribute to the dilated cardiomyopathy associated with increased PKC epsilon activity.
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