Journal
BIOCHEMISTRY
Volume 46, Issue 23, Pages 6892-6902Publisher
AMER CHEMICAL SOC
DOI: 10.1021/bi700414b
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Funding
- NCRR NIH HHS [U54 RR020839-036286, U54 RR020839] Funding Source: Medline
- NIGMS NIH HHS [R01 GM062437, R01 GM062437-08] Funding Source: Medline
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Lysine-specific demethylase 1 (LSD1) is a transcriptional repressor and a flavin-dependent amine oxidase that is responsible for the removal of methyl from lysine 4 of histone H3. In this study, we characterize the mechanism and scope of LSD1 inhibition by a propargylamine-derivatized histone H3 substrate (1). Unlike aziridinyl and cyclopropylamine-derivatized histone H3 peptide substrate analogues, compound I appears to covalently modify and irreversibly inactivate LSD1 with high potency. Accompanying this inactivation is a spectroscopic change, which shifts the absorbance maximum to 392 nm. Spectral changes associated with the 1-LSD1 complex and reactivity to decreased pH and sodium borohydride treatment were suggestive of a structure involving a flavin-linked inhibitor conjugate between N-5 of the flavin and the terminal carbon of the inhibitor. Using a C-13-labeled inhibitor, NMR analysis of the 1-flavin conjugate was consistent with this structural assignment. Kinetic analysis of the spectroscopic shift induced by 1 showed that the flavin adduct formed in a reaction with kinetic constants similar to those of the LSD1 inactivation process. Taken together, these data support a mechanism of LSD1 inactivation by I involving amine oxidation followed by Michael addition to the propargylic imine. We further examined the potential for a biotinylated analogue of I (1-Btn) to be used as a tool in affinity pulldown experiments. Using 1-Btn, it was feasible to selectively pull down spiked and endogenous LSD I from HeLa cell nuclear extracts, setting the stage for activity-based demethylase proteomics.
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