Journal
ANALYTICAL CHEMISTRY
Volume 86, Issue 23, Pages 11614-11619Publisher
AMER CHEMICAL SOC
DOI: 10.1021/ac502541v
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Funding
- National Science Foundation [CBET-1133512]
- National Institutes of Health SBIR [1R43ES022303-01]
- NATIONAL INSTITUTE OF ENVIRONMENTAL HEALTH SCIENCES [R43ES022303, R44ES022303] Funding Source: NIH RePORTER
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Surface-enhanced Raman scattering (SERS) optical nanoprobes offer a number of advantages for ultrasensitive analyte detection. These functionalized colloidal nanoparticles are a multifunctional assay component. providing a platform for conjugation to spectral tags, stabilizing polymers, and biorecognition elements such as aptamers or antibodies. We demonstrate the design and characterization of a SERS-active nanoprobe and investigate the nanoparticles biorecognition capabilities for use in a competitive binding assay. Specifically, the nanoprobe is designed for the quantification of bisphenol A (BPA) levels in the blood after human exposure to the toxin in food and beverage plastic packaging. The nanoprobes demonstrated specific affinity to a BPA aptamer with a dissociation constant K-d of 54 nM, and provided a dose-dependent SERS spectra with a limit of detection of 3 nM. Our conjugation approach shows the versatility of colloidal nanoparticles in assay development, acting as detectable spectral tagging elements and biologically active ligands concurrently.
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