Journal
ANALYTICAL CHEMISTRY
Volume 86, Issue 3, Pages 1648-1653Publisher
AMER CHEMICAL SOC
DOI: 10.1021/ac403412n
Keywords
-
Categories
Funding
- University of Manchester Research Institute (UMRI)
- EPSRC [EP/K02311X/1] Funding Source: UKRI
- Cancer Research UK [16465, 18097] Funding Source: researchfish
- Engineering and Physical Sciences Research Council [EP/K02311X/1] Funding Source: researchfish
Ask authors/readers for more resources
Fourier transform-infrared (FT-IR) chemical imaging in transmission mode has traditionally been performed on expensive mid-IR transparent windows such as barium/calcium fluoride, which are more fragile than glass, making preparation in the histopathology laboratories more cumbersome. A solution is presented here by using cheap glass substrates for the FT-IR chemical imaging, which has a high-wavenumber transmission window allowing measurement of the C-H, N-H, and O-H stretches occurring at ca. 2500-3800 cm(-1). The ''fingerprint region of the IR spectrum occurring below 1800 cm(-1) is not obtainable; however, we demonstrate that a wealth of information is contained in the high wavenumber range using 71 patients on a breast tissue microarray (TMA) as a model for investigation. Importantly, we demonstrate that the tissue can be classified into four basic tissue cell types and that using just the epithelial cells, reasonable discrimination of normal and malignant tissue can be found.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available