Journal
ANALYTICAL CHEMISTRY
Volume 86, Issue 10, Pages 4995-5001Publisher
AMER CHEMICAL SOC
DOI: 10.1021/ac500540z
Keywords
-
Categories
Funding
- National Basic Research Program of China [2013CB127801]
- Pujiang Plan of Shanghai [13PJ1407200]
- Shanghai Municipal Commission for Science and Technology [13231202800]
- BELSPO, MOST, China [S2012GR0 016]
- BELSPO, MOST, Belgium [BL/02/C58]
Ask authors/readers for more resources
A new lateral flow immunoassay (LFA) is proposed for qualitative and/or semiquantitative determination of aflatoxin B1 (AFB1), zearalenone (ZEA), deoxynivalenol (DON), and their analogues (AFs, ZEAs, DONs) in cereal samples. Each of the mycotoxin specific antibody was class specific and there was no cross reactivity to other groups of compounds. The visual limits of detection (vLOD) of the strip were 0.03, 1.6, and 10 mu g/kg for AFB1, ZEA and DON, respectively. The calculated limits of detection (cLOD) were 0.05, 1, and 3 mu g/kg respectively. Meanwhile the cutoff values were achieved at 1, 50, and 60 mu g/kg for AFB1, ZEA and DON, respectively. Recoveries ranged from 80% to 122% and RSD from 5% to 20%. Both the vLOD and CLOD for the three mycotoxins were lower than the EU maximum levels. Analysis of naturally contaminated maize samples resulted in a good agreement between the multiplex LFA and LC-MS/MS (100% for DONs and AFs, and 81% for ZEAs). Careful analysis of the results further explained the general overestimation of LFA compared to chromatographic methods for quantification of mycotoxins.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available