Journal
ANALYTICAL CHEMISTRY
Volume 86, Issue 6, Pages 2849-2852Publisher
AMER CHEMICAL SOC
DOI: 10.1021/ac403816h
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Funding
- Army Research Office [W911NF-09-2-0039]
- National Science Foundation [CHE-0955439]
- Nebraska EPSCoR [EPS-1004094]
- Direct For Mathematical & Physical Scien
- Division Of Chemistry [0955439] Funding Source: National Science Foundation
- Office Of The Director
- EPSCoR [1004094] Funding Source: National Science Foundation
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We report the use of gold-modified recessed platinum (Pt) nanoelectrodes in the fabrication of linear and stem-loop probe-based electrochemical DNA (E-DNA) sensors. Pt nanoelectrodes with a radius less than 10 nm were reproducibly fabricated using an optimized laser pulling technique. Prior to sensor fabrication, the nanoelectrode was electrochemically etched to create a recessed nanopore, followed by electrodeposition of gold into the nanopore using either cyclic voltammetry or constant potential amperometry. Both techniques enabled controlled deposition of gold into the nanopores, resulting in a nanostructured gold electrode with a well-defined surface area. In addition, we systematically determined the optimal experimental condition for DNA probe immobilization and target interrogation. The electron transfer rate constants of methylene blue, as determined using alternating current voltammetry, were found to be much higher than those obtained from E-DNA sensors fabricated on conventional macroscale electrodes. While this unique phenomenon requires further investigation, our results clearly show that these gold-modified nanoelectrodes can be used as substrates for this class of electrochemical biosensors.
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