Journal
ANALYTICAL CHEMISTRY
Volume 86, Issue 8, Pages 3869-3875Publisher
AMER CHEMICAL SOC
DOI: 10.1021/ac4040554
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Funding
- U.S. National Institute of Health [ES016865]
- National Science Foundation [CTS-0120978]
- National Natural Science Foundation of China [21175079, 21375074]
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DNA-protein conjugates are very useful in analytical chemistry for target recognition and signal amplification. While a number of methods for conjugating DNA with proteins are known, methods for purification of DNA-protein conjugates from reaction mixture containing unreacted proteins are much less investigated. In this work, a simple and efficient approach to purify DNA-invertase conjugates from reaction mixture via a biotin displacement strategy to release desthiobiotinylated DNA-invertase conjugates from streptavidin-coated magnetic beads was developed. The conjugates purified by this approach were utilized for quantitative detection of cocaine and DNA using a personal glucose meter through structure-switching DNA aptamer sensors and competitive DNA hybridization assays, respectively. In both cases, the purified DNA-invertase conjugates showed better performance compared to the same assays using unpurified conjugates. The approach demonstrated here can be further expanded to other DNA and proteins to generate purified DNA-protein conjugates for analytical and other applications.
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