Journal
DEVELOPMENTAL BIOLOGY
Volume 306, Issue 2, Pages 725-735Publisher
ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1016/j.ydbio.2007.04.009
Keywords
cyclin A1; meiosis; spermatogenesis; passenger protein complex; aurora B; spermatocyte; prophase; chromosome dynamics; chromatin; histone phosphorylation; centromere
Categories
Funding
- NICHD NIH HHS [R01 HD034915-08, R01 HD034915, HD 34915] Funding Source: Medline
Ask authors/readers for more resources
Male mice lacking cyclin A] protein are sterile. Their sterility results from an arrest in the meiotic cell cycle of spermatocytes, which we now identify as occurring at late diplotene, immediately before diakinesis. The stage of arrest in cyclin A1-deficient mice is distinct from the arrest seen in spermatocytes that are deficient in its putative catalytic partner Cdk2, which occurs much earlier in pachytene. The arrest in cyclin A1-deficient spermatocytes is also accompanied by an unusual clustering of centromeric heterochromatin. Consistent with a possible defect in the centromeric region, immunofluorescent staining of cyclin A1 protein shows localization in the region of the centromere. Phosphorylation of historic H3 at serine 10 in pericentromeric heterochromatin, which normally occurs in late diplotene, is reduced in spermatocytes from heterozygous Ccnal(+/-) testes and completely absent in spermatocytes with no cyclin A1 protein. Concomitantly, the levels of pericentromeric aurora B kinase, known to phosphorylate historic H3 during meiosis, are partially reduced in spermatocytes from testes of heterozygous mice and further reduced in homozygous null spermatocytes. These data suggest a critical and concentration-dependent function for cyclin A I in the pericentromeric region in late diplotene of meiosis, perhaps in assembly or function of the passenger protein complex. (C) 2007 Elsevier Inc. All rights reserved.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available