Decontamination of aflatoxin duckling feed with aqueous citric acid treatment

Detoxification of commercial aflatoxin B-1 (AFB(1)) contaminated feed by means of IN aqueous citric acid was confirmed by the AFLATEST (TM) immunoaffinity column method (IAC), high performance liquid chromatography (HPLC), Ames test (Salmonella-microsomal screening system), and the 1-day duckling assay. IAC and HPLC determinations showed that AFB(1) at an initial concentration of 110 ng/g was partially detoxified (86%) by the acidic treatment. Results of the Ames test indicated that the mutagenic activity of treated AFB(1) was greatly reduced (or inactivated) compared with that of untreated controls based on his(-) -> his(+) reversions in the Salmonella TAIOO strain. The toxicology and pathology studies showed that toxicity of AFB(1) was minimized by acidic treatment. The results indicated that aqueous citric acid exhibits detoxifying activity in AFB(1)-contaminated feeds and protects animals from chronic aflatoxin toxicity. (c) 2006 Elsevier B.V. All rights reserved.