Journal
ANALYTICAL CHEMISTRY
Volume 86, Issue 20, Pages 10021-10024Publisher
AMER CHEMICAL SOC
DOI: 10.1021/ac403872k
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Funding
- National Natural Science Foundation of China [11102235]
- Natural Science Foundation of Tianjin City, China [12JCZDJC24100]
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In this work, a novel enzyme-linked immunosorbent assay (ELISA) with a low limit of detection and high sensitivity was developed using atom transfer radical polymer (ATRP)-modified gold nanoparticles (AuNPs). Clear signal amplification was achieved by introducing an abundance of horseradish peroxidase (HRP) to the AuNPs, because of the ATRP modification. This result suggested that the new ELISA was able to detect antigens in complex mixtures, and the limit of detection (LOD) was lower than that of conventional ELISA by a factor of 81. The new ELISA strategy greatly decreased the LOD during analysis and exhibited excellent reproducibility, stability, and feasibility. Therefore, it is a promising technique with many potential applications in biochemistry and medical science research.
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