Journal
ANALYTICAL CHEMISTRY
Volume 86, Issue 17, Pages 8805-8813Publisher
AMER CHEMICAL SOC
DOI: 10.1021/ac502080t
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Funding
- National Science Foundation (NSF) [CBET 1337732]
- Div Of Chem, Bioeng, Env, & Transp Sys
- Directorate For Engineering [1264377] Funding Source: National Science Foundation
- Div Of Industrial Innovation & Partnersh
- Directorate For Engineering [1067943] Funding Source: National Science Foundation
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We demonstrate the first use of smartphone spectrophotometry for readout of fluorescence-based biological assays. We evaluated the smartphone fluorimeter in the context of a fluorescent molecular beacon (MB) assay for detection of specific nucleic acid sequences in a liquid test sample and compared performance against a conventional laboratory fluorimeter. The capability of distinguishing a one-point mismatch is also demonstrated by detecting single-base mutation in target nucleic acids. Our approach offers a route toward portable biomolecular assays for viral/bacterial pathogens, disease biomarkers, and toxins.
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