Journal
BIOCHEMICAL JOURNAL
Volume 404, Issue -, Pages 365-372Publisher
PORTLAND PRESS LTD
DOI: 10.1042/BJ20070081
Keywords
Candida albicans; cell-wall mannan; mannosyltransferase; nuclear-magnetic-resonance spectrum (NMR spectrum); yeast hyphal form; beta-1,2-linked mannose
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The structure of the cell-wall mannan from the J-1012 (serotype A) strain of the polymorphic yeast Candida albicans was determined by acetolysis under mild conditions followed by HPLC and sequential NMR experiments. The serotype A mannan contained beta-1,2-linked mannose residues attached to alpha-1,3-linked mannose residues and alpha-1,6-linked branching mannose residues. Using a beta-1,2-mannosyltransferase, we synthesized a three-beta-1,2-linkage-containing mannoheptaose and used it as a reference oligosaccharide for H-1-NMR assignment. On the basis of the results obtained, we derived an additivity rule for the H-1-NMR chemical shifts of the beta-1,2-linked mannose residues. The morphological transformation of Candida cells from the yeast form to the hyphal form induced a significant decrease in the phosphodiesterified acid-labile beta-1,2-linked manno-oligosaccharides, whereas the amount of acid-stable beta-1,2-linkage containing side chains did not change. These results suggest that the Candida mannan in candidiasis patients contains beta-1,2-linked mannose residues and that they behave as a target of the immune system.
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