Journal
JOURNAL OF CHROMATOGRAPHY A
Volume 1153, Issue 1-2, Pages 74-89Publisher
ELSEVIER
DOI: 10.1016/j.chroma.2007.01.028
Keywords
sample treatment; carbohydrates; fractionation; derivatization; clean-up; chromatographic techniques
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Carbohydrates are a very important group of compounds due to their roles as structural materials, sources of energy, biological functions and environmental analytes; they are characterized by their structural diversity and the high number of isomers they present. While many advances have been made in carbohydrate analysis, the sample preparation remains difficult. This review aims to summarize the most important treatments which have been recently developed to be applied prior to the analysis of carbohydrates by chromatographic techniques. Due to the multiplicity of structures and matrices, many different techniques are required for clean-up, fractionation and derivatization. A number of new techniques which could be potentially adequate for carbohydrate characterization have also been revised. (c) 2007 Elsevier B.V. All rights reserved.
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