Journal
JOURNAL OF BIOLOGICAL CHEMISTRY
Volume 282, Issue 24, Pages 17501-17506Publisher
AMER SOC BIOCHEMISTRY MOLECULAR BIOLOGY INC
DOI: 10.1074/jbc.M701770200
Keywords
-
Categories
Funding
- NIGMS NIH HHS [GM070891, GM043974] Funding Source: Medline
Ask authors/readers for more resources
ATM ( ataxia-telangiectasia mutated) is necessary for activation of Chk1 by ATR ( ATM and Rad3-related) in response to double-stranded DNA breaks ( DSBs) but not to DNA replication stress. TopBP1 has been identified as a direct activator of ATR. We show that ATM regulates Xenopus TopBP1 by phosphorylating Ser-1131 and thereby strongly enhancing association of TopBP1 with ATR. Xenopus egg extracts containing a mutant of TopBP1 that cannot be phosphorylated on Ser-1131 are defective in the ATR-dependent phosphorylation of Chk1 in response to DSBs but not to DNA replication stress. Thus, TopBP1 is critical for the ATM-dependent activation of ATR following production of DSBs in the genome.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available