Journal
BIOSENSORS & BIOELECTRONICS
Volume 22, Issue 12, Pages 3283-3287Publisher
ELSEVIER ADVANCED TECHNOLOGY
DOI: 10.1016/j.bios.2007.03.006
Keywords
urea; activity; urease immobilization; urea biosensor; nanoporous alumina membranes
Ask authors/readers for more resources
The urease was immobilized onto nanoporous alumina membranes prepared by the two-step anodization method, and a novel piezoelectric urea sensing system with separated porous alumina/urease electrode has been developed through measuring the conductivity change of immobilized urease/urea reaction. The process of urease immobilization was optimized and the performance of the developed urea biosensor was evaluated. The obtained urea biosensor presented high-selectivity monitoring of urea, better reproducibility (S.D. = 0.02, n = 6), shorter response time (30 s), wider linear range (0.5 mu M to 3 mM), lower detection limit (0.2 mu M) and good long-term storage stability (with about 76% of the enzymatic activity retained after 30 days). The clinical analysis of the urea biosensor confirmed the feasibility of urea detection in urine samples. (c) 2007 Elsevier B.V. All rights reserved.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available