Journal
ANALYTICAL CHEMISTRY
Volume 86, Issue 19, Pages 9866-9872Publisher
AMER CHEMICAL SOC
DOI: 10.1021/ac502496h
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Funding
- National Basic Research Program of China (973 Program) [2011CB911002]
- National Natural Science Foundation of China [21075119]
- Open Project of the State Key Laboratory of Supramolecular Structure and Materials [SKLSSM20141.5]
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Superquenching of aggregation-induced emission (AIE) has been utilized in biosensing for the first time. A positively charged tetraphenylethene derivative (compound 1) showed no emission in an aqueous buffer solution. A single-stranded DNA (a polyanion) induced aggregation of compound 1, and strong compound 1 aggregate emission was observed. When the DNA was labeled with a quencher molecule, compound 1 aggregate emission was efficiently quenched. On the basis of this observation, a new, simple, sensitive and selective DNA methyltransferase (MTase) assay has been developed. A quencher-labeled double-stranded DNA could induce aggregation of compound 1, and superquenching of compound 1 AIE was observed. In the presence of MTase and an endonuclease, the DNA could be specifically methylated and cleaved into single-stranded DNA fragments. The quencher molecule was released, and a turn-on emission signal was detected.
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