Journal
ANALYTICAL CHEMISTRY
Volume 86, Issue 19, Pages 9853-9859Publisher
AMER CHEMICAL SOC
DOI: 10.1021/ac502453z
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Funding
- National Institutes of Health [1 R01 GM112048-01A1, 1DP20D007209-01]
- Huck Innovative & Transformational Seed Fund
- Penn State Center for Nanoscale Science (MRSEC) [DMR-0820404]
- NIAID, NIH [AI45818]
- NSF
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Precise reconstruction of heterotypic cellcell interactions in vitro requires the coculture of different cell types in a highly controlled manner. In this article, we report a standing surface acoustic wave (SSAW)-based cell coculture platform. In our approach, different types of cells are patterned sequentially in the SSAW field to form an organized cell coculture. To validate our platform, we demonstrate a coculture of epithelial cancer cells and endothelial cells. Real-time monitoring of cell migration dynamics reveals increased cancer cell mobility when cancer cells are cocultured with endothelial cells. Our SSAW-based cell coculture platform has the advantages of contactless cell manipulation, high biocompatibility, high controllability, simplicity, and minimal interference of the cellular microenvironment. The SSAW technique demonstrated here can be a valuable analytical tool for various biological studies involving heterotypic cellcell interactions.
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