Journal
ANALYTICAL CHEMISTRY
Volume 86, Issue 22, Pages 11013-11017Publisher
AMER CHEMICAL SOC
DOI: 10.1021/ac503453v
Keywords
-
Categories
Funding
- National Science Foundation [ECCS-0335765]
- Cornell Center for the Microenvironment and Metastasis [U54 CA143876]
Ask authors/readers for more resources
Limited access to tumor tissue makes repeated sampling and real-time tracking of cancer progression infeasible. Circulating tumor cells (CTCs) provide the capacity for real-time genetic characterization of a disseminating tumor cell population via a simple blood draw. However, there is no straightforward method to analyze broadscale genetic rear-rangements in this heterogeneous cell population at the single cell level. We present a one-step controllable chemical extraction of whole nuclei from prostate cancer cells captured using geometrically enhanced differential immunocapture (GEDI) microdevices. We have successfully used copy number profile analysis to differentiate between two unique cancer cell line populations of metastatic origin (LNCaP and VCaP) and to analyze key mutations important in disease progression.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available