Journal
ANALYTICAL CHEMISTRY
Volume 86, Issue 5, Pages 2332-2336Publisher
AMER CHEMICAL SOC
DOI: 10.1021/ac5001256
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Funding
- U.S. National Institutes of Health [R01 CA120792]
- U.S. National Science Foundation [CHE-0911092]
- Division Of Chemistry
- Direct For Mathematical & Physical Scien [0911092] Funding Source: National Science Foundation
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The concentration of human scrum albumin (HSA) indicates the health state of individuals and is routinely measured by UV spectroscopy with bromocresol. However, this method tends to overestimate HSA, and more critically, depends highly on the timing, in seconds, of the measurements. Here, we report an analog of 2',7'-dichlorofluorescein that can be used as a fluorescent sensor to quantify HSA in human sera. The accuracy of this new method proved superior to that of bromocresol when an international standard scrum sample was analyzed. This method is more convenient than the bromocresol method because it allows for fluorescence measurements during a >15 min period. Colorimetric analysis was also performed to further investigate the effects of the binding of the sensor to HSA. These spectroscopic studies suggest that absorption and emission changes upon HSA binding may be due to the dehydration of the dye and/or stabilization of the tritylic cation species.
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