Journal
EMBO JOURNAL
Volume 26, Issue 12, Pages 2966-2980Publisher
NATURE PUBLISHING GROUP
DOI: 10.1038/sj.emboj.7601717
Keywords
C. elegans; cilia; forkhead domain; intraflagellar transport; kinesin
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Funding
- NCRR NIH HHS [S10 RR 16708] Funding Source: Medline
- NIGMS NIH HHS [R37 GM056223, R01 GM056223, GM 56223] Funding Source: Medline
- NINDS NIH HHS [P30 NS045713, P30 NS 45713] Funding Source: Medline
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Individual cell types can elaborate morphologically diverse cilia. Cilia are assembled via intraflagellar transport (IFT) of ciliary precursors; however, the mechanisms that generate ciliary diversity are unknown. Here, we examine IFT in the structurally distinct cilia of the ASH/ASI and the AWB chemosensory neurons in Caenorhabditis elegans, enabling us to compare IFT in specific cilia types. We show that unlike in the ASH/ASI cilia, the OSM-3 kinesin moves independently of the kinesin-II motor in the AWB cilia. Although OSM-3 is essential to extend the distal segments of the ASH/ASI cilia, it is not required to build the AWB distal segments. Mutations in the fkh-2 forkhead domain gene result in AWB-specific defects in ciliary morphology, and FKH-2 regulates kinesin-II subunit gene expression specifically in AWB. Our results suggest that cell-specific regulation of IFT contributes to the generation of ciliary diversity, and provide insights into the networks coupling the acquisition of ciliary specializations with other aspects of cell fate.
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