Journal
ANALYTICAL CHEMISTRY
Volume 86, Issue 22, Pages 11373-11379Publisher
AMER CHEMICAL SOC
DOI: 10.1021/ac5033319
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Funding
- National Basic Research Program of China [2011CB933502]
- National Natural Science Foundation of China [21335004, 21427807]
- Natural Science Foundation of Anhui Province [1408085MF114]
- China Postdoctoral Science Foundation [2013M541637]
- Jiangsu Province Postdoctoral Science Foundation [1302126C]
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In this work, electrogenerated chemiluminescence resonance energy transfer (ECL-RET) between luminol as a donor and CdSe@ZnS quantum dots (QDs) as an acceptor was reported in neutral conditions. It was observed that a glassy carbon electrode modified with CdSe@ZnS quantum dots (CdSe@ZnS/GCE) can catalyze the luminol oxidation to promote the anodic luminol ECL without coreactants. The intensity of anodic luminol ECL (0.60 V) at the CdSe@ZnS/GCE was enhanced more than 1 order of magnitude compared with that at the bare GCE. Another stronger anodic ECL peak observed at more positive potential (1.10 V) could be assigned to the ECL-RET between the excited state of luminol and the QDs. A label-free ECL aptasensor for the detection of thrombin was fabricated based on the synergic effect of the electrocatalysis and the ECL-RET. The approach showed high sensitivity, good selectivity, and wide linearity for the detection of thrombin in the range of 10 fM-100 pM with a detection limit of 1.4 fM (S/N = 3). The results suggested that the as-proposed luminol-QDs ECL biosensor will be promising in the detection of protein.
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