Journal
ANALYTICAL CHEMISTRY
Volume 86, Issue 4, Pages 2246-2250Publisher
AMER CHEMICAL SOC
DOI: 10.1021/ac404103r
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Funding
- National Basic Research Priorities Program [2012CB910602, 2013CB911201]
- National Natural Science Foundation of China [21075022, 20875017, 21105016]
- Research Fund for the Doctoral Program of Higher Education of China [20110071110007, 20100071120053]
- Shanghai Municipal Natural Science Foundation [11ZR1403200]
- Shanghai Leading Academic Discipline Project [B109]
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Abnormal protein glycosylation has been demonstrated to be associated with many diseases; therefore, it is very important to conduct a comprehensive structure analysis of glycan for prognosis and diagnosis of diseases, such as cancer. In this work, for the first time, carbon-functionalized ordered graphene/mesoporous silica composites (denoted as C-graphene@mSiO(2)) with large surface area and uniform pore size were designed and synthesized. By taking advantage of the special interaction between the carbon and glycans as well as size-exclusion ability, 25 N-linked glycans released from ovalbumin were observed clearly with strong MS signals and increased signal-to-noise (S/N) ratio. In addition, after enrichment with the C-graphene@mSiO(2) composites, 48 N-linked glycans (S/N > 10) with sufficient peak intensities were obtained from only 400 nL of healthy pristine human serum. The facile and low-cost synthesis method as well as high selective enrichment ability of the novel C-graphene@mSiO(2) composite makes it a promising tool for glycosylation research.
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